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2013 AVMA Euthanasia guidelines.

Discussion about fish health and water chemistry.

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Post Tue Apr 29, 2014 4:46 pm
abmcdonald Level 4 Member
Level 4 Member

Posts: 141
Location: Shakopee
Any one know what the correct concentration is for using clove oil to euthanize 2.5 - 3.5 inch rift lake cichlids?
How does one ensure clove oil is properly mixed with the water, being that oil and water don't generally mix all that well?

Post Tue Apr 29, 2014 5:44 pm
Passionfish Level 20 Member
Level 20 Member

Posts: 12161
Location: apple valley, mn
abmcdonald wrote:
Any one know what the correct concentration is for using clove oil to euthanize 2.5 - 3.5 inch rift lake cichlids?
How does one ensure clove oil is properly mixed with the water, being that oil and water don't generally mix all that well?


Create an emulsion by vigourous shaking or blending oil in water. The recommended concentration of 400 mg/L is expected to elicit respiratory failure.
Rather than adding 400 mg to a L and then passing this through a blender, one can place 150 mg in 100 ml of water. Shake 100 ml oil and water until an emulsion forms. An emulsion is tiny droplets of oil dispersed throughout water phase. Emulsion will appear cloudy or similar to a suspension (powder suspended in liquid).
Place fish in 1 gal of water, add 100 ml emulsion and stir slowly to disperse emulsion. Wait 10-15 min and repeat. Wait an additional 10-15 min and repeat again. At this point there will be enough clove oil in water to elicit respitory failure. If fish is still moving gills, do not add more clove oil. Gently stir water and wait until clove oil takes effect.

BTW, active ingredient in clove oil is eugenol and isoeugenol. These two ingredients should comprise 85-95% of the purchased clove oil. 150 mg of clove oil is 0.15 ml by volume.
Like a complete unknown

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